Long-term preserved mycelium establishes the presence of Cryphonectria carpinicola in the Balkans and of Cryphonectria radicalis in Bulgaria

Abstract Around the world, isolate collections in mycological institutes have long preserved valuable specimens and information for science. In present study, we determine Cryphonectria -like taxonomic rarities our 10–24 years old collection from Balkans to link dry mycelium with DNA data. Using ITS sequences, confirm first time occurrence of carpinicola on a Carpinus tree extend range radicalis found Castanea sativa Bulgaria. The oldest specimen examined dates 1998 molecularly confirms reported finding North Macedonia.